Journal: Journal of Biological Chemistry
Article Title: Platelet-derived Growth Factor-BB and Basic Fibroblast Growth Factor Directly Interact in Vitro with High Affinity
doi: 10.1074/jbc.m108858200
Figure Lengend Snippet: FIG. 4. Binding of different proteins to immobilized bFGF an- alyzed by SPR. Anti-bFGF antibody (100 g/ml), PDGF-BB (500 nM), aFGF (500 nM), vitronectin (500 nM), BSA (500 nM), and fibronectin (500 nM) were injected onto immobilized bFGF. Different RU responses indicate different binding of each protein to bFGF. Results represent the average S.D. of 3 experiments.
Article Snippet: Recombinant human PDGF-BB and acidic FGF (aFGF) were purchased from R & D Systems (Abingdon, UK); recombinant human bFGF, fibronectin, and vitronectin were purchased from Invitrogen; elastase was purchased from Worthington; bovine serum albumin fraction V (BSA) was purchased from Sigma; blotting grade blocker nonfat dry milk was from Bio-Rad; 125I-bFGF and Hybond ECL membranes were purchased from Amersham Biosciences AB.
Techniques: Binding Assay, Injection